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One such method is the mass spectrometric immunoassay (MSIA), which involves micro-scale immunoaffinity-based sample purification and enrichment, followed by mass spectrometric detection

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Int J Androl 13:1725

The brains were fixed with 4% paraformaldehyde (PFA) in phosphate buffered saline (PBS) at 4 C for 24 h and cryopreserved with 30% sucrose