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38,39 Although its often claimed that dietary arginine is necessary for detoxifying ammonia, researchers in 2008 found that ammonia was adequately detoxified with endogenous arginine and without any dietary arginine.40 In fact, studies with healthy adults have revealed that production of arginine in the body remains unchanged even if arginine and arginine precursors like glutamate are eliminated from the diet.41 Importantly, the 2008 study showed that in the absence of dietary arginine, degradation of arginine and oxidation of arginine to potentially harmful inflammatory NO were markedly reduced. 40 Dietary arginine actually adds disproportionately to the amount of nitrogen needing detoxification

Molecules 21 (9), 1136

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Thus it is vital to follow a specific process within 30 minutes of collection: Collect blood plasma (using EDTA or heparin to prevent coagulation) and then centrifuge the sample to remove the blood cells from the plasma