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Fecal DNA extraction The fecal samples were collected by restraining the mice with a single hand and collected with 1.5 ml eppendorf

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The sealed container slows oxygen ingress but doesnt prevent hydrolysiswater in the formulation allows peptide bond cleavage even without external contamination

General Characteristics of Adipose Tissue Adipose tissue is a critical regulator of metabolic homeostasis, functioning as an energy depot and an endocrine organ capable of secreting biofactors (called adipokines) which regulate appetite, body fat distribution, insulin sensitivity and secretion, energy expenditure, and inflammation [86]

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