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Interscapular brown adipose tissue was homogenized in Tris-ethylenediaminetetraacetic acid (EDTA) buffer (10 mM Tris and 1 mM EDTA, pH 7.4) with 1% protease inhibitor (Nacalai Tesque, Kyoto, Japan) on ice

Categorical variables were reported as frequencies and percentages, and continuous variables were summarized as means and standard deviations or medians with interquartile ranges depending on the data distribution

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PLoS ONE 14:e0214347 Brudziak P, Panuszko A, Kaczkowska E, Piotrowski B, Daghir A, Demkowicz S, Stangret J (2018) Taurine as a water structure breaker and protein stabilizer