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Mass spectrometric analyses were conducted using a Q Exactive Orbitrap mass spectrometer (Thermo Fisher Scientific, USA) in negative ion mode at a resolution of 140,000 (m/z 100500)

In animal models, the elevated TMAO levels were significantly related to tubulointerstitial fibrosis and collagen deposition in the kidney and TMAO was demonstrated to increase in kidney injury molecule-1, phosphorylation of Smad3 (Tang et al., 2015b)

[DOI] [PubMed] [Google Scholar] 155.Sappington D.R., Siegel E.R., Hiatt G., Desai A., Penney R.B., Jamshidi-Parsian A., Griffin R.J., Boysen G

doi: 10.1016/j.pharmthera.2015.11.011 95 HaruwakaK.IkegamiA.TachibanaY.OhnoN.KonishiH.HashimotoA.et al