This gives you peace of mind, knowing that your peptide solution will stay clean and safeeven with multiple uses from the same vial
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Boc-L-Glu-O t Bu was dissolved in THF in the presence of BOP, Castros reagent as the coupling reagent, di-isopropylethylamine (DIEA) as the base, and finally the protected amino acid hydrochloride H-L-Glu (O t Bu)-O t Bu, HCl was added
If improvements plateau after three to four months, stepping up to 2% may reinvigorate progress