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For the preparation of mitochondrial subfractions, 1 mg of isolated mitochondria was suspended with 40 l T10E20 buffer (10 mM Tris-HCL, 1 mM EDTA, pH 7.6) and then incubated with digitonin (0.1 mg digitonin/mg of mitochondria protein) for 10 min on ice

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PRRT using [111In-DTPA]-octreotide (where DTPA is diethylenetriamine pentaacetic acid) is feasible because, besides gamma radiation, 111In emits both therapeutic Auger and internal conversion electrons having tissue penetration ability [39, 40]

Temperature excursions above 8C for more than 4 hours trigger irreversible denaturation the peptide may appear clear but has lost potency