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Briefly, frozen tissue was powdered with a nitrogen-cooled mortar and pestle, and 50-70mg of tissue were homogenized in the absence (for GSH determination) or presence (for GSSG determination) of 1-methyl-2-vinylpyridinium trifluoromethanesulfonate to scavenge reduced thiols

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Although Lurcher mice do not have any significant difference in latency before falling in the wooden beam test, they have been seen to spend more time walking than WT mice over repeated trials, which is most likely a strategy to regulate and maintain body equilibrium to prevent them from falling [119, 137]

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