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Slides were then mounted on a temperature-controlled stage in a VisiScope CSU-X1 spinning disk confocal system (Visitron Systems) equipped with a Plan-Apochromat 63x/1.4 oil objective on an AxioObserver.Z1 microscope (Zeiss), a Evolve 512 EMCCD Camera (Photometrics), and the VisiView v4.00.10 Imaging software (Visitron Systems)