Kongo partners with U.S.-based compounding pharmacies that are NABP-accredited and LegitScript-verified to provide medications containing semaglutide, the same active ingredient found in Wegovy and Ozempic
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[DOI] [PMC free article] [PubMed] [Google Scholar] 145.Aksoy D, et al
To confirm whether recombinant proteins of AsGGT1, AsGGT2, and AsGGT3 were expressed as mature GGT enzymes in yeast cells, we first examined deglutamylation activities of these recombinant proteins by using the standard procedure that utilizes -glutamyl- p -nitroanilide, a common synthetic -glutamyl donor substrate for known GGTs ( p -nitroanilide to p -nitroaniline ( Table 1 ), showing that yeast endogenous GGT could utilize -glutamyl- p -nitroanilide as a -glutamyl donor substrate, as reported previously ( p -nitroaniline released from -glutamyl- p -nitroanilide in assays using crude protein extracts from yeast expressing AsGGT1, AsGGT2, and AsGGT3, respectively, were significantly higher than that in assays using crude protein extracts from control yeast ( Table 1 ), indicating that the recombinant proteins of AsGGT1, AsGGT2, and AsGGT3 were successfully expressed and folded to form mature functional GGT proteins that can utilize -glutamyl- p -nitroanilide as a -glutamyl donor substrate in yeast cells
Meier TB, Drevets WC, Teague TK, Wurfel BE, Mueller SC, Bodurka J, et al
