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Warfarin sodium was purchased from Tokyo Chemical Industry Co

In animal-model work, NNMT inhibition with 5-Amino-1MQ has been associated with changes in adipose-tissue mass, in NAD+ pool sizes (because NNMT consumes nicotinamide that would otherwise feed the NAD+ salvage pathway), and in methyl-donor dynamics (because NNMT consumes S-adenosylmethionine)

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1000 g solubilized protein extracted from rat striatal tissue was incubated in the presence of anti-D2R antibody (Proteintech Group, rabbit, catalogue# 550841-AP) or control IgG (Merck Millipore, rabbit, catalogue# 12370), together with protein A/G plus agarose (Santa Cruz Biotechnology) overnight at 4 C