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Antibodies employed in this study were specific for the following molecules: CD3 (clone OKT3 mouse mAb), CD28 (clone 28.2, BD Pharmingen), isotype control antibodies IgG1 and IgG2a (mouse mAb, BD Biosciences), cysteine sulfenic acid (rabbit polyclonal antibody, Merck), Prx1 (peroxiredoxin1, rabbit polyclonal antibody, Invitrogen), Trx (thioredoxin, Clone 2G11, mouse mAb, BD Bioscience), phospho-STAT3 (pSTAT3, #9131, Cell Signaling Technology), STAT3 (#9139, Cell Signaling Technology), and GAPDH (clone 6C5, mouse mAb, Invitrogen)

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The comet assay data from the identified publications were subsequently entered into a Microsoft Access database

FIGURE 7 Quantitative gene expression analysis revealed significant alterations in Keap1 and AMPK levels across the experimental groups