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This was shown in HEK 293 cells, known to have voltage-gated K + [272,273], Na + [274], and Ca 2+ [275] channels, and with different local anesthetics applications (i.e., bupivacaine [89], lidocaine [60], tetracaine [276], and oxibuprocaine [276])

Researchers studying gastrointestinal effects have used both acute (single-dose) and chronic (7-14 day) protocols, with efficacy observed in both paradigms

Proc Natl Acad Sci USA 1991

In animal research, early angiogenic and lesion-reduction markers appear within days, with connective-tissue phases playing out over roughly 4-8 weeks