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doi: 10.1172/JCI129484 66 MahajanATaliunDThurnerMRobertsonNRTorresJMRaynerNWet al

Gastroenterology 147 , 577594.e571 (2014)

Companion satellite-cell cultures, prepared at the same time, were immunostained at 30-h post-plating with D3 anti-desmin mAb and DAB (a chromogenic HRP-substrate that forms an insoluble brown reaction product) to determine the percentage of myogenic cells present 18 ,19 cultures with less than 95% DAB-positive cells (namely, less than 95% desmin-positive myogenic purity) were not used for experiments just to ensure the reliability of data specific to myogenic cells in the employed primary cell culture systems

The deconvoluted spectrum is reported on the Certificate of Analysis alongside the HPLC data, so the 16-residue chain and the N-terminal tyrosine substitution can both be confirmed against expected values before reconstitution