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(PubMed) Mazokopakis EE, Starakis IK

If a validated research protocol requires frozen solution storage, aliquots should be prepared under controlled conditions so each portion is thawed once rather than repeatedly freezing and thawing the same vial

Of course, there are certain situations where you should talk to your doctor about the possibility of needing more vitamin B12if you are vegan (meaning you dont eat animal products), are over 60, or have a condition or take a medication that could deplete your B12 levels, leading to a variety of symptoms (see below)

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