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In line, Grx2 knockout mice resemble phenotypes of mice expressing a hypomorphic Gpx4 allele, such as lipid peroxidation in the brain or loss of parvalbumin-positive neurons [71]

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Frozen cortical samples (the weights of which were determined beforehand) were homogenized in 5% 5-sulfosalicylic acid (SSA), and the mixtures were centrifuged at 8000 g for 10 min at 4 C