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Murine organoids were grown in crypt culture medium (CCM), advanced DMEM/F12 medium (Gibbco) containing 2 mM GlutaMax (Gibbco), 10 mM HEPES, penicillin, streptomycin and amphotericin (all Sigma- Aldrich) supplemented with N2, B27 (both Gibbco), 1 mM N-acetylcystein (Sigma- Aldrich), 50 g/mL EGF (ImmunoTools), 100 g/mL noggin and 0.5 g/mL R-spondin 1 (both PeproTec) or, when derived from colonic crypts, in Murine Intesticult medium (STEMCELL Technologies)
A full list of potential side effects is available in the patient information leaflet provided with your medication
Once carefully injected by our expert practitioners into specific problem areas, this solution initiates a biochemical reaction within the adipose tissue
Use at your own risk and follow proper dosage guidelines