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Western blot analysis The left SN (SNpc + SNr) was washed with ice-cold PBS and lysed in radioimmunoprecipitation assay buffer composed of 50 mM Tris-HCl (pH 7.4), 150 mM sodium chloride, 1% Nonidet P-40, and 0.1% sodium dodecyl sulfate (SDS) containing phosphate and protease inhibitor cocktails

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In the reduced state, the thiol group of cysteine is able to donate an electron to other molecules