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The column oven temperature was 50 C, the mobile phase consisted of acetonitrile-methanol-water (30:16:54 v/v, pH 45 adjusted by 0.01 N HCl), the flow rate was 1.2 ml/min and the eluate absorbance was monitored online at a wavelength of 230 nm

The cuproptosis-related gene PDHA1 may serve as a node linking these pathways, regulating lipid metabolism through its interaction with the PI3K-Akt-mTOR signaling pathway [123] (Figure 5)

However, it is important to recognise that the relative contribution of each compound is far more complex and that additive and synergistic effects cannot be ruled out