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Examination of the GST-GFP reporter, which contains multiple anti-oxidant response elements (ARE) and is activated in oxidized tissue 38 , shows robust activations in APAP-exposed animals (Fig

Finally, soluble nuclear lysate was diluted with two volumes of the dilution buffer (60 mM HEPES-KOH (pH = 7.9), 1 mM DTT, 30 % glycerol, 0.4 % NP-40, 1 x phosphatase inhibitor cocktail (Sigma, P0044) and 1 x protease inhibitor cocktail (Sigma, P8340)) to lower KCL concentration to 150 mM