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The principle of this reaction is based on the reaction between the thiol group (-SH) of glutathione and the DTNB reagent, which results in the formation of a colored product

Glutathione is a peptide derived from three amino acids: glutamate, cysteine, and glycine

These systems metabolize and scavenge for reactive oxygen species, in a controlled and precise fashion

Actually, let me reframe that

Approximately 2% of the oxygen consumed by the mitochondria is converted into superoxide, making it one of the largest sources of endogenous ROS ( via -oxidation of fatty acid and flavin oxidase reaction and degrading ROS via catalase-mediated breakdown of H 2 O 2 ( Table 1 Formation of major oxidants