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Traditional cigarette smoke consists of microscopic airborne ash particulates, vaporized nicotine isolates, and gas combustion by-products

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After washing the membranes four times with TBST buffer, they were incubated at room temperature for 2 h with anti-His tag antibody (1:2000, Abbkine) in TBST buffer

The chromatograms were recoded and integrated with the Galaxie data system (Varian Inc.)