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The GST-fusion protein first binds to glutathione agarose, and then after other contaminating proteins have been washed away, reduced glutathione is added to elute the GST-fusion protein from the resin (Figure 5)

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Pros and Cons Pros: Effective for dark spots Safer than hydroquinone Cons: Limited effect on overall dullness Safety and Combination Safe for long-term use and can be combined with glutathione-based regimens

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