Here's a pro tip: angle the needle so the tip is against the inside wall of the vial
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The Antioxidant Cycle Most antioxidants, like Vitamin C or Vitamin E, perform their job and then become "spent." They give up an electron to neutralise a free radical and can no longer help until they are "recharged." Glutathione is unique because it can actually help recycle these other antioxidants
Superoxide radical anion scavenging assay The superoxide anion (O ) scavenging activity assay is a widely used method to evaluate the ability of antioxidants to neutralize superoxide anions, a highly ROS implicated in oxidative stress and cellular damage (Carocho & Ferreira, 2013)