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When working with an eukaryotic expression system that may contain unknown agents and factors interfering the binding of target protein, it is recommended to dilute the culture supernatant 1:1 or 1:2 with the Equilibration buffer (for example, 20 mM sodium phosphate, 0.5 M NaCl, pH 7.4)

The importance of early avoidance or treatment of sepsis, anti-inflammatory strategies, nutritional support and subsequent rehabilitation is emphasized in previous studies

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Recent studies revealing differential roles for the two estrogen receptors in skin biology and pathology have opened exciting new avenues for therapeutic intervention