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of AZT the nanoparticles encapsulating the AZT-TP are recovered by centrifugation (10000 rpm / 10 min, at ambient temperature) and dried under vacuum for 3 days

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2.8.4 Determination of carotene by HPLC-PDA The sample (0.5 g) was added to a centrifuge tube (50 mL), 30 mL of petroleum ether was added, shaken gently, ventilated, capped, and shaken for 10 min at room temperature, and the supernatant was separated