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Delineating the effects of elevated circulatory FFA concentrations on -cell function and viability remains a significant challenge, due to factors such as between-participant variability in plasma FFA levels/composition and -cell secretory capacity through influences such as ethnicity, sex and genetic background

We fixed 8-m muscle cryosections with 3% paraformaldehyde, pH 7.3 in 1 PBS, washed in 1 PBS, permeabilized nuclei using 0.5% Triton X-100 in 1 PBS for 5 min or 0.2% Triton X-100 in 1 PBS for 10 min at room temperature, and incubated in pre-hybridization solution (30% formamide/2 SSC) for 10 min at room temperature

"The role of acetaldehyde in human psychomotor function: a double-blind placebo-controlled crossover study"

C.DingH.GiorgadzeN.et al (2015)