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The plates were then replenished with 10 mM pyruvate + 1 mM malate or 5 mM glycerol-3-phsophate + 2 M rotenone in 1 MAS buffer supplemented with 4 mM ADP and 1 nM XF-PMP

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The day before the experiment, 25,000 cells/well were seeded in eight-well chambers (Thermo Scientific)

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The proteasome is part of the ubiquitin proteasome system (UPS), which is critical for protein quality control and is the main intracellular degradation pathway that eliminates polyubiquitinated misfolded and oxidized proteins by a multi-complex 26 S proteasome 16,17,24,40