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Methods Human bronchial epithelial cell isolation and culture Human bronchial epithelia were isolated from main stem/lobar bronchi of human excess donor and excised recipient normal lung by enzymatic digestion following protocol #031396 approved by the University of North Carolina Biomedical Institutional Review Board 45

Take spaced-out puffs rather than long drags

Those staring, staring eyes

When doing this option I would recommend following it up with a wipe around the inside of the mouthpiece and chimney just to make sure that there is no lingering e-liquid

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