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After 24 h, the biofilm was washed once with 1 PBS and imaged using phase contrast microscopy (Zeiss, Axio, Germany), for bacterial adhesion and colonization

Analyzing real-time PCR data by the comparative C-T method

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Signs of intramuscular injection include deeper, more intense pain during injection, increased bruising, faster onset of side effects (nausea, headache appearing within hours instead of the typical timeline), and a sore, tender feeling in the thigh muscle for a day or more after injection

(2004) Proc Natl Acad Sci U S A 101 : 13163 PubMed: 15333749 Search on PubMedSearch on PubMed Central DOI: Primary Citation Related Structures: 1USB PubMed Abstract: A strategy for rational enzyme design is reported and illustrated by the engineering of a protein catalyst for thiol-ester hydrolysis