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35 On day 8, after the mice were weighed and anesthetized, a 100 L dose of 2.5% (w/v) TNBS in 50% ethanol was administered intrarectally via a catheter inserted 4 cm into the colon, with the mice maintained in a head-down position for 5 min

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To be accounted as reasons behind the constant growth in prevalence of E-cigarette use there are the believes that E-cigarettes are less harmful than traditional tobacco cigarettes, that they could help tobacco-smokers quit the smoking habit and the several flavours that represent one of the most appealing factors to adolescents

Acknowledgments We would like to thank our country-level subject matter experts who were instrumental in identifying overtly feminine packs: Geni Achnas and Olga Knorre from Campaign for Tobacco Free Kids

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