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Magnetic beads were washed four times with PBS/BSA and finally resuspended in DMEM/F12 (Invitrogen, Carlsbad, CA, #11320033) medium containing 20% FBS, endothelial cell growth factor (Sciencell, Faraday Ave, Carlsbad, #1052), 100 units/mL penicillin, and streptomycin and plated on gelatin-coated 6-well plates