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Samples related to the calibration curve were processed in the same manner adopted for the biological samples

Data Transparency All data and materials support published claims and comply with field standards

Hydroxyl radicals are extremely reactive and may cause extensive damage to cellular components

Lifestyle modifications include: Beginning regular exercise Developing good sleeping patterns/habits Reducing the use of stimulants Reducing the consumption of alcohol and tobacco Non-lifestyle treatment methods include: Leg massages Hot baths and/or ice packs A vibrating pad In some cases drugs may be used, but the effects may relieve or worsen your RLS and the effectiveness may lessen over time