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Darmstadt, Germany) or Honeywell (Seelze, Germany), formic acid and ammonium bicarbonate for LC-MS from Honeywell, dithiothreitol (DTT) from Pierce Biotechnology (Rockford, IL, United States), iodoacetamide from Sigma-Aldrich (St

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Gradual Introduction: Start with lower concentrations (e.g., 0.05% to 1%) and less frequent application (e.g., every other day) to allow the skin to acclimate, especially for sensitive skin types