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These sudden surges of overwhelming fear and anxiety can strike without warning, leaving individuals feeling helpless and desperate for answers

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Some specific details related to substance use should be asked including: start date, last use, method of use (aerosol, dabbing or dripping), duration of use, frequency of puffs, and concomitant tobacco or other drug use

Perspectives on Psychological Science, 6 (6), 589609

The OD value of the obtained solution was 0.2 at 408 nm by a microplate reader, which represented 100% concentration and was diluted to the desired concentration with non-FBS culture medium