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Genetic heterogeneity Southern hybridisation of genomic DNA isolated from selected clones of lines 1/7 and 1/3 clearly documented that even the round-shaped primary calli can contain cell populations with different T-DNA insertions

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Removing it, you should be able to see an empty reservoir

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This may suggest that individuals with deteriorating health might increase their smoking behavior due to emotional regulation needs or difficulties associated with withdrawal, or it could indicate reverse causality (e.g