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Plotting the fluorescence intensity ratios with excitation wavelengths at 405 and 488 nm (F 405 /F 488 ) as a function of GSH concentrations confers a superb linear relationship ( R 2 =0.998) covering the physiological GSH concentration range 110 mM (Fig

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However, mitochondria are devoid of GSH synthesis, whose uptake depends on special transporters

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