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2.8 Semi-native gel electrophoresis 5-L reaction samples or 10 g of purified VLPs were supplemented with 6x gel loading purple dye (NEB) prior to separation by 1% (w/v) agarose gel electrophoresis for 60 min at 60 V in TAE buffer (40 mM Tris base, 20 mM acetic acid, 1 mM EDTA) supplemented with 0.3 g/mL ethidium bromide (Roth)