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On the AX12, AX iAECs were maintained in AX Alveolar Epithelial Barrier Medium (AlveoliX, Switzerland) supplemented with 1% penicillinstreptomycin (Thermo Fisher Scientific, Switzerland) for mono-culture studies and AX iAECs/hLMVECs were maintained in AX E2 Alveolar Barrier Medium (provided by AlveoliX) for all dual-culture studies

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Resting: Take time for regular breaks, whether its through naps, reading, playing games, or watching a favorite show