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Samples were then extracted and cleaned according to AOAC Method 2007.01 for pesticide residues in foods by acetonitrile extraction and partitioning with magnesium sulphate (REFERENCE: available online at Ragworm Samples: Fifty milligram ragworm samples were weighed into 2 mL tubes and spiked with internal standard solution (10 L of a solution containing 500 ng/mL nicotine-D4 and cotinine-D3) together with 300 L water (2% ammonium hydroxide) and 400 L acetonitrile

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Waste Disposal: Cigarette manufacturing can produce waste and by-products that need to be disposed of properly, which may incur additional costs

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