Compared to reversed-phase high performance liquid chromatography (RP-HPLC), HIC is a gentle method that helps maintain the biological activities of sample molecules, leading to its wide applications for biomolecule separation, especially for protein species
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Compounds 34 and 35 (Figure 25) were found to be the most effective inhibitors of P38 kinase, with IC 50 values of 47 nM and 45 nM, respectively