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After freeze-thaw processing, supernatants were collected and stored at -80C until required ( 2.2 Cell viability assay Cell Counting Kit-8 (CCK-8) (Beyotime Biotech, China) was used to measure the corresponding optical density ratio marker cell viability levels in 96-well plates according to the manufacturers instructions

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This is the critical threshold you must never exceed for any extended period

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Keywords: Endoscopic surgery