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While Nicotine is the main addictive substance in tobacco smoke, many major smoking-related diseases are associated with toxicants produced by combustion, including carbon monoxide and carcinogenic compounds in smoke

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When NO donor was added to tobacco induced cells, development of partial cell deaths was observed which confirmed the role of NO as the cell death mediator Biomarkers Major nicotine metabolite Cotinine 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanol (NNAL) Essentiality and deficiency (specific to metals) : N/A References Engstrom P.F., Clapper, M.L and Schnol, R.A