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We will measure YAP expression and colocalization with markers of reactive astrocytosis (GFAP, LCN2, Serpin 3N) using immunostaining and confocal imaging

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On day 28 the rats were exposed to air/smoke in the morning and in the afternoon the rats were challenged with vehicle (saline, aerosolised for 30 min) or OVA (1% w / v , aerosolised for 30 min)

Theres a lot more to the processes and chemistry than this space will allow, and my hands-on experience with some of the methods is minimal, but I hope that this has made things a bit easier to understand

Makhachkala is about 1,600 kilometers (990 miles) south of Moscow