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Quantitative PCR To analyze mRNA expression, total RNA was collected from cells using the PureLink RNA Mini Kit (Thermo Fisher Scientific), and 1 g was reverse-transcribed using the ProtoScript II First Strand cDNA Synthesis Kit with oligo(dT) primers and murine RNase inhibitor (New England Biolabs)

Multi-compound workflows require careful planning

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Methodology Under all aseptic precautions 10 ml of morning blood sample was collected from anticubital vein of controls & cases after an overnight fasting