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Background As discussed in section II.A.1

Figure 1 Decreased RNA expression levels of immune checkpoints, such as TIGIT, CTLA-4, and BTLA were observed in epithelium obtained from nasal scrape biopsies of self-described active e-cigarette users compared with samples from non-smokers ( In vitro analysis indicated that exposure of human neutrophil to e-cigarette aerosols impaired migration, phagocytosis, oxidative burst response, extracellular trap formation, and induced changes in PD-L1 expression

Despite these increases in coverage, by the end of 2021, less than half of the U.S