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Each piece is in exceptional, perhaps even unused condition

The respiratory system acts as a natural filter, with different regions capturing particles based on their aerodynamic properties: Upper Airways (Throat and Trachea): Capture particles larger than 5 micrometers First line of defense against larger contaminants Associated with irritation and inflammation responses Large Airways (Bronchi): Trap particles between 1-5 micrometers Can trigger bronchial responses and coughing Important for understanding chronic bronchitis development Small Airways and Alveoli: Receive particles smaller than 1 micrometer Critical for gas exchange function Most vulnerable to long-term damage from fine particles Research shows that the majority of cigarette and e-cigarette aerosol mass falls within the 0.4 to 1.3 micrometer range, meaning these particles can penetrate deep into the lungs where they may cause the most significant health impacts

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To best create these translatable pre-clinical animal models, it is necessary to standardize factors such as puff frequencies and topographies, exposure concentrations, e-liquid composition and device generation/brand to ensure consistent E-cigarette Vapour Deposition

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