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Notably, also scoring in HEK-293T cells was Thioredoxin Reductase 2 (TXNRD2), a component of the thioredoxin (Trx) dependent antioxidant system specifically localized to mitochondria (Fig 3c

Pretreated cells were washed with PBS, followed by incubation with 1 ml of JC-1 working solution per sample at 37 C for 20 minutes

1 2 Kanzok SM, Fechner A, Bauer H, Ulschmid JK, Mller HM, Botella-Munoz J, Schneuwly S, Schirmer R, Becker K (2001)

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Patients with oral malignant melanoma often recall having an existing oral pigmentation months to years before diagnosis, and the condition may even have elicited prior comment from physicians or dentists