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PubMed 26236730 Pickart L

Cells were thereafter lysed with Lysis Buffer #6 (R&D Systems) and assayed for the expression levels of phospho-ERK1 (T202/Y204)/ERK2 (T185/Y187) and phospho-JNK with commercial kits (DuoSet IC ELISA, R&D Systems, # DYC1018B & DYC1387B) according to manufacturers instructions

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It is evident that RPM supplementation not only altered the seminal plasma composition toward an improvement in semen functionality but also influenced epigenetic regulation