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In vitro evaluation of intraluminal factors that may alter intestinal permeability in ponies with carbohydrate-induced laminitis

Transepithelial resistance measurements Caco-2 cells were seeded onto 12 mm polycarbonate membrane inserts (PCF membrane inserts, Millipore, Germany) and bathed in DMEM/F12 medium which contained phenol red, 10% FBS, penicillin (100 units/ml) and streptomycin (10 g/ml)

2,150,151 The Par complex is localized to tight junctions of endothelial cells by binding PAR-3 to JAM proteins